NutriFreez D5 염 기반 냉동 보존 솔루션은 세포 치료 및 임상 응용을 위한 세포의 동결 및 해동을 위해 화학적으로 정의되고
특별히 설계된 최적의 올인원 솔루션입니다.
초저온(-196°C) 환경에서 정의된 보호 환경을 제공하여 매우 민감한 세포에서도 높은 생존율, 회복 속도 및 성능을 보장합니다.
모든 로트는 광범위한 QC 테스트의 일환으로 MSC에서 테스트되지만,
NutriFreez D5는 hPSC, PBMC, NK, Vero 세포 등 다양한 다른 세포에서도 널리 테스트되었습니다.
NutriFreez D5는 cGMP 조건에서 제조되었으며 업계 최고의 성능을 제공합니다.
이 제품은 최적의 제형으로, 5% DMSO를 함유하고 있으며 항생제, 항진균제, 호르몬, 성장 인자, 혈청 또는 단백질을 전혀 포함하지 않습니다.
Differentiation potential of cryopreserved AT-MSCs into the adipogenic,
chondrogenic and osteogenic lineages Representative images of the differentiated
AT-MSCs originating from a donor’s MSCs, cryopreserved in NutriFreez D5 Salt Based Solution.
The cells were thawed at P2 and for passage P3/P4 seeded and grown in adipogenic,
chondrogenic and osteogenic differentiation medium (BI),
in order to evaluate the cryopreserved AT-MSCs stemness (Bourin et al., 2013).
The cells were stained as follows: Adipocytes were stained on day 10 of adipogenic differentiation
with Oil Red O Stock to visualize lipids and fat deposits (A);
In osteocytes the calcium deposits in the cells were stained with Alizarin Red S on day 18-21 of osteogenic differentiation (B);
in chondrocytes the sulfated proteoglycan present in cartilage tissue is stained on day 21 of chondrogenic differentiation with Alcian Blue (C).
Attribute | |||
---|---|---|---|
Volume (ml) | 10 ml | 100 ml | 500 ml |
% DMSO | 5 | 5 | 5 |
QC Cells | hMSC | hMSC | hMSC |
Item | Description | Package | Volume | Quantity | Size | Order Number |
---|---|---|---|
NutriFreez D5 | A Salt Based Protein Free, 5% DMSO Cryopreservation Solution |
10 ml | 05-715-1D |
NutriFreez D5 | A Salt Based Protein Free, 5% DMSO Cryopreservation Solution |
100 ml | 05-715-1B |
NutriFreez D5 | A Salt Based Protein Free, 5% DMSO Cryopreservation Solution |
500 ml | 05-715-1A |
Product Name | Description | Description | Order Number |
---|---|---|---|
Dulbecco’s PBS (w/o Ca & Mg) | ![]() |
A buffer solution | 02-023-1 |
Soybean Trypsin Inhibitor (SBTI) | ![]() |
A trypsin inhibitor solution | 03-048-1 |
Cell dissociation solution – non enzyamtic | ![]() |
A non-enzymatic cell dissociation solution | 03-071-1 |
MSC NutriStem XF Basal Medium | ![]() |
A media for the expansion of MSCs | 05-200-1 |
MSC NutriStem Supplement Mix | ![]() |
Supplementation for NutriStem Basal Medium | 05-201-1 |
MSC Attachment Solution | ![]() |
A solution for the attachment of hMSCs on culture plates | 05-752-1 |
Recombinant Trypsin Solution | ![]() |
A solution for the dissociation of adherent cells | 03-078-1 |
Recombinant Trypsin-EDTA Solution | ![]() |
A solution for accelerated dissociation of adherent cells | 03-079-1 |
NutriFreez D10 Cryopreservation Medium | ![]() |
A solution for cryopreservation of cells (10% DMSO) | 05-713-1 |
NutriStem hPSC XF Medium | ![]() |
A media for the expansion of hPSCs | 05-100-1 |