미등록페이지

Figure : 1 NutriStor 냉동 보관 용액에 저장된 세포와 NutriFreez D10 냉동 보존 배지 간의 비교.

Sample 1.

Cells after storage in NutriStor®.
Initial seeding: 44.0×10³ cells/cm²

Sample 2.

Cells after storage in NutriStor®.
Initial seeding: 44.8×10³ cells/cm²

Positive control

Cells after cryopreservation in NutiFreez® D10.
Initial seeding: 41.8×10³ cells/cm²

Note: Representative images (×100) of human BM-MSCs after a 3-day storage in NutriStor Cold Storage solution. After storage, the cells were seeded for recovery with
MSC NutriStem XF Medium. Cells were counted and images were taken 3 days post-seeding.
The cells stored in NutriStor Cold Storage Solution showed impressive results, with high recovery and proliferation rates, and normal morphology.

Figure : 2 CFU-F 분석. 냉장 보관 후 hMSC의 자가 재생 가능성 비교

Note: Representative images (×40) of mature BM-MSC colony forming units, stained with 0.5% Crystal violet. Images were taken post 14 days of CFU-F assay.
After storage in cold storage solution, cells were seeded at a concentration of 100 cells/well, to assess colony-forming potential.
Cells stored in NutriStor Cold Storage Solution showed much higher colony-forming potential than Leading competitor. Morphology was similar in NutriStor
and PC wells. Similar results were obtained while testing AT/CT and DP-derived MSCs.

Figure : 3 타 브랜드와의 PBMC 배양 생존가능성 및 회복성 비교

A)

Volume	NutriStor Cold Storage Solution	Leading competitor’s solution	NutriFreez D5 Cryopreservation Solution (PC)
Donor 1
Donor 2

B)

Note: Fresh cells from two healthy donors were stored in NutriStor Cold Storage Solution or in the leading competitor's solution, for 4 days,
at 4°C, 5-6*10^6 cells/mL per vial [1 mL/vial]). NutriFreez D5 cryopreservation solution served as a positive control. The cells were seeded post-storage in
Nutri-T XF medium in 24 well plates, at a concentration of 50×10³ cells/well, and cultured for 8 days with a passage on day 5.
(A) Light microscopy images taken on day 5 post-seeding.
PBMCs stored in NutriStor Cold Storage Solution or PC showed typical culture morphology and higher proliferation rates than the competitor’s solution.
(B) Cells were counted, and fold expansion (FE) was evaluated as a recovery indicator on day 8.
Though viability results were good in both solutions, PBMCs stored in NutriStor showed higher viability and significantly higher FE results than the
cells stored in the competitor’s solution.