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mRNA | Adherent & Suspension & Hard-to-transfect | Gene expression, IPSC generation, reprogramming, stem cell differentiation, immunotherapy assays
Reagent | jetMESSENGER® |
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Molecule delivered | mRNA |
Applications | Gene expression |
Cell types | Hard-to-transfect cells |
Number of transfections | 1.5 ml of jetMESSENGER® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates |
Storage | Store jetMESSENGER® at 5 °C ± 3°C. |
Provided with | mRNA buffer |
Take your gene expression to the next level by switching to mRNA!
Primary cells, neurons, suspension cells and various cancer cell lines are especially challenging to transfect. jetMESSENGER® gives high transfection efficiency in all of these usually difficult to transfect cells, by allowing highly efficient mRNA transfection.
mRNA transfection is as easy as DNA transfection, with the advantage that mRNA does not need to reach the cell nucleus for expression nor require cell division for efficient gene expression. Hence, cells that are slow dividing or that have developed specific mechanisms to protect their genome can finally be used for gene expression.
Unravel the full potential of your cells by switching to mRNA transfection.
A video presenting mRNA transfection for hard-to-transfect cells is available
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA (Jove)
Reference Number | Amount of reagent | Amount of buffer |
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101000056 | jetMESSENGER® 0.1 mL | 10 ml |
101000005 | jetMESSENGER® 0.75 mL | 60 ml |