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Virl vector manufacturing
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상품 정보
상품 기본설명
AAV | RUO / GMP | Large scale rAAV manufacturing
상품 상세설명
| Reagent | FectoVIR®-AAV |
|---|---|
| Molecule delivered | Plasmid DNA and co-delivery of several plasmid DNA |
| Applications | Recombinant AAV virus production in suspension cells |
| Virus types | Adeno-Associated Viruses (AAV) |
| Cell types | Mammalian producer cell lines (HEK-293, HEK-293 derivatives) grown in suspension cultures |
| Number of transfections | 100 mL of FectoVIR®-AAV transfection reagent is sufficient to transfect on average 50 L of cell culture |
| Storage | Store FectoVIR®-AAV at 5 °C ± 3°C. |
| Provided with | Protocol |
Summary
AAVs are becoming the leading viral vector in the development of gene-based advanced therapy medicinal products (ATMPs). Their success is due to their intrinsic properties: they are primarily non-integrating as they persist within cells as episomes, and have a broad tropism with eleven serotypes that have the ability to infect specific cell types. A major challenge today is manufacturing sufficient quantities of AAV in order to treat larger groups of patients. FectoVIR®-AAV is a novel class of animal free transfection reagent specifically developed for industrial scale production of recombinant AAV (rAAV) viral vectors in suspension HEK-293 cell types. FectoVIR®-AAV transfection reagent guarantees higher rAAV viral titers, improved flexibility and scalability for industrial scale manufacturing.
A Frequently Asked Question section about FectoVIR®-AAV can be found here.
Ordering information
| Reference Number | Amount of reagent |
|---|---|
| 101000044 | FectoVIR®-AAV 1 mL |
| 101000022 | FectoVIR®-AAV 10 mL |
| 101000004 | FectoVIR®-AAV 100 mL |
| 101000054 | FectoVIR®-AAV 1 L |
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