Use Mini-PROTEAN Spacer Plates with short plates to form cassette sandwiches when casting polyacrylamide gels. These plates can be used for casting gels with a Mini-PROTEAN Tetra System Casting Frame and Stand, or a Mini-PROTEAN 3 Multi-Casting Chamber.
IPTG (isopropyl-beta-D-thiogalactopyranoside) is an analog of lactose. It inactivates the lac repressor and induces the synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. This product is a 50 mg/ml solution by dissolving 250mg of IPTG in 5 ml of distilled H2O. IPTG is used to induce the expression of cloned genes under the control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.
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