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Dead Cell Removal Kit

The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes.

★ Single cell suspension에서 부터 myelin debris를 효과적으로 제거가능

★ Microbeads를 샘플과 incubation을 한 뒤, column을 이용한 depletion 방식으로 myelin 제거

Overview

The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes.

Detailed product information

Applications

The Dead Cell Removal Kit has been successfully used for the following applications:

* Removal of dead cells prior to MACS® Separation of cells

* Reduction of flow sorting time by elimination of dead cells

* Elimination of dead cells from cell cultures

* Improvement of immunocytochemical analysis of frozen cells

* Preparation of viable, single-cell suspensions from tissue

* Removal of dead cells from spermatozoa samples

* Removal of dead cells prior to or following transfection of cells

Columns

MS, LS, XS, or autoMACS® Columns.

Figure 1

Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide.

Figure 2

Working scheme for the Dead Cell Removal Kit.