시약

★ 정제된 단백질로부터 endotoxin과 DNA를 쉽고 빠르게 제거
★ Beads에 공유 결합된 polycationic ligand는 Endotoxins과 DNA의 polyanionic molecules과 강하게 결합되는 윈리로 제거

Specifications for Endotoxin Removal Beads

Overview

Endotoxin Removal Beads represent the ideal tool for fast and easy removal of endotoxin and DNA from purified protein preparations.

Preparations of recombinant proteins are often contaminated with endotoxins (lipopolysaccharides, LPS) and DNA from host bacteria. Bacterial endotoxins and DNA show strong biological effects in many mammals as well as in cell cultures. If purified recombinant proteins are used for in vivo or in vitro assays, efficient removal of endotoxins and bacterial DNA is required to avoid non-specific detrimental effects.

The covalently bound polycationic moiety of the Endotoxin Removal Beads shows strong binding to both endotoxins and DNA.

Detailed product information

Background information

Endotoxin or DNA can be removed by 98% at initial concentrations ranging from 2000 EU/mL to 10,000 EU/mL or 2000 to 10,000 ng of DNA per mL, respectively. Endotoxin removal is performed using the MACSiMAG™ Separator.

Note: Endotoxin Removal Beads are not suitable for use with MACS® Columns and MiniMACS™, MidiMACS™, VarioMACS™, SuperMACS™, autoMACS®, μMACS™, or thermoMACS™ Separators.

Figure 1

Removal of various endotoxins from a 1.0 mg/mL BSA solution in 0.1 mol/L sodium-phosphate buffer. The Endotoxin Removal Bead–concentration was 15 mg/mL. BSA recovery was above 84% at any rate.

Figure 2

Correlation of endotoxin removal and protein recovery. The Endotoxin Removal Bead concentration was 15 mg per mL of 0.1 M Na2HPO4 buffer. The incubation time was 10 minutes.