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Myelin Removal Beads II have been developed for the specific removal of myelin debris from single-cell suspensions. Myelin Removal Beads II work without determination of cell number or myelin debris/cell ratio.
★ Cell culture 또는 조직 준비에서 dead cell을 빠르게 제거가능
★ Microbeads과 binding buffer를 이용 하여 쉽게 제거가능
Specifications for Myelin Removal Beads II, human, mouse, rat
Overview
Myelin Removal Beads II have been developed for the specific removal of myelin debris from single-cell suspensions. Myelin Removal Beads II work without determination of cell number or myelin debris/cell ratio.
Detailed product information
Background information
When myelin-containing neural tissue is dissociated, large quantities of myelin debris are generated, which considerably impair antibody binding.1,2
Depletion of myelin debris from single-cell suspensions leads to improved immunostaining and cell separation results.
Columns
LS or autoMACS® Columns.
Figure 1
Mouse brains (P28) were dissociated using the Neural Tissue Dissociation Kit (P). Flow cytometric analysis shows that the resulting single-cell suspension contains large amounts of myelin membrane fragments and other cellular debris. Only 3% of the signals represent cells (A). Myelin Removal Beads II used with an LS Column and a MidiMACS Separator effectively remove myelin debris (B).